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| Crego-vicente, Beatriz; Fernández-soto, Pedro; Febrer-sendra, Begoña; García-bernalt Diego, Juan; Boissier, Jérôme; Angora, Etienne K.; Oleaga, Ana; Muro, Antonio. |
| Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed... |
| Tipo: Text |
Palavras-chave: LAMP; Schistosomiasis; Schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; Molecular diagnosis; Species-specific LAMP; Genus-specific LAMP. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00686/79840/82659.pdf |
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| Aradaib,Imadeldin Elamin; Abbas,Babiker; Osburn,Bennie; Bushara,Hamid Omer; Taylor,Martin. |
| Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Schistosoma bovis; Vaccine. |
| Ano: 1994 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020 |
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| Angora, Etienne K.; Allienne, Jean-francois; Rey, Olivier; Menan, Herve; Toure, Andre O.; Coulibaly, Jean T.; Raso, Giovanna; Yavo, William; N'Goran, Eliezer K.; Utzinger, Jurg; Balmer, Oliver; Boissier, Jerome. |
| Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Cote d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Cote d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman(R) FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A... |
| Tipo: Text |
Palavras-chave: Cote d'Ivoire; Hybrids; Molecular analysis; Schistosoma bovis; Schistosoma haematobium. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73604/73060.pdf |
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| Djuikwo-teukeng, Felicite Flore; Simo, Alain Kouam; Allienne, Jean-francois; Rey, Olivier; Ngapagna, Arouna Njayou; Tchuem-tchuente, Louis Albert; Boissier, Jerome. |
| BackgroundSchistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present novel data on the population genetic structure of Schistosoma bovis, a highly widespread and prevalent schistosome infecting ruminants, and therefore of veterinary importance.MethodsAdult S. bovis were sampled in the two main abattoirs of Cameroon (Yaounde and Douala). Twenty-two cows originating from four distinct localities were sampled and a total of 218 parasites were recovered. All parasites were genotyped using a panel of 14 microsatellite markers and a... |
| Tipo: Text |
Palavras-chave: Schistosoma bovis; Barcoding; Population genetic structure. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00481/59267/62448.pdf |
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